Clin Immunol, Vol 126, pp 180-188, 2008
Decrease of CD4+CD25highFoxp3+ regulatory T cells and elevation of CD19+BAFF-R+ B cells and soluble ICAM-1 in myasthenia gravis
Li X, et al
Myasthenia gravis (MG) is caused by T-cell-dependent autoantibodies
against muscle acetylcholine receptors (AChR) at the neuromuscular
junction. Here, we adopted ELISA and flow cytometry techniques to
measure the levels of Th1, Th2, Th3 cytokines, inflammatory cytokine
and chemokine sICAM-1 and to analyze the phenotypes of CD4+ and CD8+ regulatory cells as well as the expression of BAFF-R on CD19+
B cells in peripheral blood from 75 MG patients and 50 healthy
controls. There were no differences in the levels of IL-2, IL-4, IL-10,
IL-13, IFN-γ, TNF-α, TGF-β and sCTLA-4 in both sera and culture
supernatants between MG patients and healthy controls. The level of
IL-12 was decreased in culture supernatants from MG patients, and the
level of sICAM-1 was increased in both sera and culture supernatants
from MG patients. Although the populations of CD8+CD28− and CD8+CD122+ regulatory T cells were not different between MG patients and healthy controls, MG patients exhibited the decrease of CD4+CD25highFoxp3+ regulatory T cells and the increase of CD19+BAFF-R+ B cells, revealing that MG patients should display the dysfunction of T cell balance and the activation of B cell maturation.
