Core Facilities

Every Immune Tolerance Network (ITN) trial integrates a set of genetic, cellular and immunological studies that are designed to explore the underlying mechanisms of tolerance and disease. They help to identify new clinical phenotypes by stratifying individuals in the clinical trials, and to discover new biomarkers that may illuminate drug targets or be useful for personalizing therapy. To accomplish this, the ITN has developed the following core laboratories and facilities that utilize state-of-the-art and custom-made assays.

Distributed Centers of Excellence

Allergy & Asthma

Principle Investigators: Professor Stephen Durham and Dr. Mohamed Shamji | Imperial College, London, UK

Transplant

Principle Investigator: Professor Allan Kirk | Emory University, Atlanta GA 

Diabetes

Principle Investigators: Dr. Jane Buckner and Dr. Alice Long | Benaroya Research Institute, Seattle, WA

 Core Laboratories

DNA Sequencing Core

Protocol Chair:
Harlan Robins, Adaptive Biotechnologies, Seattle, WA

Adaptive Biotechnologies performs immune profiling of T cell and B cell receptors using ImmunoSEQ technology.

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DNA Methylation Core

Protocol Chair:
Ulrich Hoffmeuller, Epiontis, Berlin, Germany

Epigenetic analysis of immune cells is performed at Epiontis GmbH, a biotechnology company located in Berlin, Germany.

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Immunological Diagnostic Testing Core

Protocol Chair:
Steven Kleiboeker, ViracorIBT, Lee's Summit, MO

ViracorIBT is a CLIA-certified, molecular and immunological diagnostics laboratory with approximately 16,000 sq. ft. of laboratory space located in Lee’s Summit, MO (within the Kansas City metropolitan area).

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Hepatitus A Titer Assessment Core

Protocol Chair:
Lutz Prizdun, Mediagnost, Reutlingen, Germany

Mediagnost Anti-HAV IgG ELISA E10 is well suited to show immunity to hepatitis A virus.

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Cytokine Assessment Core

Protocol Chair:
Scott Van Arsdell, Aushon BioSystems, Billerica, MA

Aushon BioSystems operates a CLIA certified testing laboratory in Billerica, MA and provides a comprehensive list of 300 validated protein biomarker assays.

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Type 1 Diabetes Autoantibody Core

Protocol Chair:
Robert Allan, University of Florida, Gainesville, FL

The University of Florida, Pathology Laboratory, and Endocrine Autoantibody Laboratory performs islet cell autoantibody testing for the Immune Tolerance Network.

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Central Cell Isolation Core

Protocol Chair:
Michael Sheldon, Rutgers University, Piscataway, NJ

The Rutgers University Cell & DNA Repository serves as the Immune Tolerance Network's Central Cell Isolation Facility.

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MHC-Peptide Multimer Core

Protocol Chair:
Gerald Nepom, Benaroya Research Institute, Seattle, WA

New MHC Class II tetramer reagents and staining procedures are being developed in facilities at Benaroya Research Institute in Seattle, WA.

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Tissue Pathology Core at UPMC

Protocol Chair:
Anthony Demetris, University of Pittsburgh, Pittsburgh, PA

The Division of Transplantation Pathology at the University of Pittsburgh Medical Center offers a comprehensive consultation service in solid organ transplantation pathology to community and university based physicians.

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Flow Cytometry Core

Protocol Chair:
Paul Wallace, Roswell Park Cancer Institute, Buffalo, NY

The Department of Flow & Image Cytometry at Roswell Park Cancer Institute serves as a core facility for the Immune Tolerance Network, providing comprehensive flow cytometric immunophenotyping services.

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Alloantibody and Flow Crossmatch Core

Protocol Chair:
Robert Bray, Emory University, Atlanta, GA

This core provides state-of-the-art testing for HLA antibodies.

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Type 1 Diabetes Autoantibody Core

Protocol Chair:
Liping Yu, University of Colorado, Aurora, CO

The Immune Tolerance Network Autoantibody Core analyzes patient serum for the presence of autoantibodies using semi-automated, high-throughput radioassays.

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Gene Microarray Core

Protocol Chair:
Steve McPhail, Expression Analysis, Inc., Durham, NC

The Immune Tolerance Network (ITN) has established a microarray core designed to evaluate global patterns of gene expression using the Affymetrix GeneChip array technology.

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HLA-Typing Core

Protocol Chair:
Rajalingam Raja, UCSF, San Francisco, CA

The primary goal of the HLA Typing Core is to provide sequence level typing using DNA collected from participants in ITN trials.

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