Core Facilities

Every Immune Tolerance Network (ITN) trial integrates a set of genetic, cellular and immunological studies that are designed to explore the underlying mechanisms of tolerance and disease. They help to identify new clinical phenotypes by stratifying individuals in the clinical trials, and to discover new biomarkers that may illuminate drug targets or be useful for personalizing therapy. To accomplish this, the ITN has developed the following core laboratories and facilities that utilize state-of-the-art and custom-made assays.

Distributed Centers of Excellence

Allergy & Asthma

Principle Investigators: Professor Stephen Durham and Dr. Mohamed Shamji | Imperial College, London, UK

Transplant

Principle Investigator: Professor Allan Kirk | Duke University, Durham, NC 

Diabetes

Principle Investigators: Dr. Jane Buckner and Dr. Alice Long | Benaroya Research Institute, Seattle, WA

Core Laboratories

High-Throughput Immunosequencing Core

Principal Investigator:
Harlan Robins, Adaptive Biotechnologies, Seattle, WA

Adaptive Biotechnologies performs immune profiling of T cell and B cell receptors using ImmunoSEQ technology.

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Epigenetic Immune Monitoring Core

Principal Investigator:
Ulrich Hoffmeuller, Epiontis, Berlin, Germany

Epigenetic analysis of immune cells is performed at Epiontis GmbH, a biotechnology company located in Berlin, Germany.

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Immunological Diagnostic Testing Core

Principal Investigator:
Steven Kleiboeker, ViracorIBT, Lee's Summit, MO

ViracorIBT is a CLIA-certified, molecular and immunological diagnostics laboratory with approximately 16,000 sq. ft. of laboratory space located in Lee’s Summit, MO (within the Kansas City metropolitan area).

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Central Cell Isolation Core

Principal Investigator:
Michael Sheldon, Rutgers University, Piscataway, NJ

The Rutgers University Cell & DNA Repository serves as the Immune Tolerance Network's Central Cell Isolation Facility.

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MHC-Peptide Multimer Core

Principal Investigator:
Gerald Nepom, Benaroya Research Institute, Seattle, WA

New MHC Class II tetramer reagents and staining procedures are being developed in facilities at Benaroya Research Institute in Seattle, WA.

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Tissue Pathology Core at UPMC

Principal Investigator:
Anthony Demetris, University of Pittsburgh, Pittsburgh, PA

The Division of Transplantation Pathology at the University of Pittsburgh Medical Center offers a comprehensive consultation service in solid organ transplantation pathology to community and university based physicians.

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Type 1 Diabetes Autoantibody Core

Principal Investigator:
Liping Yu, University of Colorado, Aurora, CO

The Immune Tolerance Network Autoantibody Core analyzes patient serum for the presence of autoantibodies using semi-automated, high-throughput radioassays.

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HLA-Typing Core

Principal Investigator:
Rajalingam Raja, UCSF, San Francisco, CA

The primary goal of the HLA Typing Core is to provide sequence level typing using DNA collected from participants in ITN trials.

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