Type 1 Diabetes Autoantibody Core

Location: University of Colorado Health Science Center, Denver, CO

Overview | Investigators | Background | Resources

Overview

The ITN Autoantibody Core analyzes patient serum for the presence of autoantibodies
using semi-automated high throughput radioassays. Translated cDNA constructs are used to
express autoantigens for the assay. Currently, there are three anti-islet autoantibody tests
(GAD65, ICA512 and mIAAA), one Addison’s disease related autoantibody test (21-
hdryoxlyase) and one Celiac disease-related autoantibody test (tissue transglutaminase). New
assays are in development. All of these assays can be run with Ig isotyping (IgG subclasses,
IgA, IgE, and IgM). The blood samples for autoantibody tests are collected in red
top (or tiger-top) tubes.

Participating Investigators

George Eisenbarth, University of Colorado Health Science Center, Denver, CO

Background Articles

	Enumerating autoreactive T cells in peripheral blood: a big step in diabetes prediction - J Clin Invest [go]
	High-throughput radioassays for autoantibodies to recombinant autoantigens - Front Biosci [go]
	One third of HLA DQ2 homozygous patients with type 1 diabetes express celiac disease-associated transglutaminase autoantibodies - J Autoimmun [go]

Resources & Interesting Links

	Type 1 Diabetes: Molecular, Cellular and Clinical Immunology - Barbara Davis Center [go]
	Combinatorial Islet Autoantibody Workshop Results- Immumology of Diabetes Society [go]