Boutique Real Time PCR Core

Locations: Harvard University, Boston, MA
New York Presbyterian Hospital (Cornell), New York, NY

Overview | Investigators | Background

Overview

Real-time PCR Cores, located at Harvard University (Director, Terry Strom, M.D.) and Cornell University (Director, M. Suthantiran, M.D.) are supported by the ITN to perform real-time PCR analyses on peripheral blood, urine and tissue for several genes implicated in acute rejection, tolerance or tolerance breakdown.

These genes include, but are not limited to granzyme B, cyclooxygenase, perforin, IL-10, IL-7 and IL-15. Quantitative PCR is performed to assess relative expression levels of these genes between normal controls and patients, and in single patients at various timepoints. These intent is to to track such parameters as disease onset, disease progression and patient responses to therapeutics.

Participating Investigators

	Terry Strom, Beth Israel Deaconess Medical Center, Harvard University
	Manikkam Suthanthiran, Cornell University Medical Center

Background Articles

	Quantification of mRNA using real-time RT-PCR: trends and problems - J Mol Endocrinol [go]
	Real-time multiplex PCR assays - Methods [go]
	An overview of real-time quantitative PCR: applications to quantify cytokine gene expression - Methods [go]