 |
Enzyme
Linked ImmunoSpot (ELISPOT) Assay Core
Location: Case Western Reserve
University, Cleveland, OH
Overview | Investigators
| Background | Resources
The ITN ELISPOT Core provides analyses of frozen cell
samples from patient peripheral blood for secreted cytokine
assays. These analyses include quantitation of secreted
INF-gamma, Il-2,4,5, and 10 in response to alloantigen
stimulation. Principal Investigators determine the selection
of cytokines and antigens to be used in the ELISPOT assay
in conjunction with the Core and Tolerance Assay Directors.
For studies in transplantation, the
ELISPOT core facility will perform assays using donor
spleen or peripheral blood cells as stimulators and
appropriately matched cells from 3rd party donors as
controls. In addition, the facility can use peptides
derived from the polymorphic regions of donor HLA molecules
to assess reactivity through the indirect pathway.
For studies of patients with Type-I Diabetes, the facility
uses a panel of overlapping 15mer peptide sequences
from the GAD65 protein as antigen. In the future, the
ITN Subgroups or Steering committee will define other
antigens to be added to the ELISPOT core standard panels.
 Participating
Investigators
|
Paul Lehman, Case
Western Reserve University |
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Peter Heeger, Cleveland
Clinic Foundation |
Background
Articles
|
|
Direct Visualization
of Cytokine-Producing Recall Antigen-Specific CD4
Memory T Cells in Healthy Individuals and HIV Patients
- J Immunol [go ] |
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Pretransplant
Frequency of Donor-Specific, IFN- -Producing ...correlates
with the Risk of Posttransplant Rejection Episodes
- J Immunol [go] |
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ELISPOT analysis of
donor-reactive immunity posttransplantation as
a clinically useful immune monitoring tool - Am
J Transplantation [go] |
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Single-Cytokine-Producing
CD4 Memory Cells Predominate in Type 1 and Type
2 Immunity - J Immunol [go] |
Resources
& Interesting Links
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Cellular
Technology, Ltd. [go] |
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