ITN - Resources



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ASSAY PROTOCOLS

The ITN provides the standard operating procedures and protocols, developed by ITN personnel and collaborators, free to use by the research community. Please note that no technical support is available. All works are licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 2.5 License.

RNA Preparation/QC

RNA 002

Total RNA Isolation From Blood Collected Into a ABI Tempus™ Whole Blood Collection tube

Lab Protocol

v.008

RNA 003

Concentration of isolated RNA using Millipore Microcon Centrifugal DeviceYM-100

Lab Protocol

v.009

RNA 004

Assessing the quality of RNA using the Agilent 2100 Bioanalyzer

Lab Protocol

v.003

Flow Cytometry

RPCI 001

In vitro Intracellular Cytokine Staining With and Without Stimulation

Lab Protocol

v.002

RPCI 003

EMA Staining

Lab Protocol

v.002

RPCI 004

Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method)

Lab Protocol

v.002

Other

PBMC CPT

Isolation and Cryopreservation of PBMC – CPT without Plasma Collection

Lab Protocol

v.009

LIST OF TOLERANCE-RELATED GENES

The ITN, in cooperation with Applied Biosystems and Celera Genomics, has compiled a list of 1000 genes believed to have specific relevance to immune tolerance and the immune response.

The list inclides known genes and ESTs suspected to be involved in immune regulation, including genes for cytokines/chemokines, cell surface receptors, apoptosis proteins, signal transduction molecules, transcription factors, heat shock proteins, extracellular proteins and nuclear receptors.

The following spreadsheet contains a detailed list of the genes:

ITN_ToleranceGeneList.xls (MS Excel; 189kb) - right click to download

Probe-primer pairs for all sequences in the list are available for purchase from Applied Biosystems Assays-on-Demand Service.