Central Cell Isolation Core

The Rutgers University Cell & DNA Repository (RUCDR) serves as the Immune Tolerance Network's Central Cell Isolation Facility. The role of the core is to receive and process human blood and tissue specimens for isolation, cell viability analysis, cryopreservation, cryogenic storage and shipping of isolated mononuclear B cell fractions isolated from whole blood, spleen and lymph node tissues. RUCDR is the  largest university-based repository in the world.

References: 

1. Jindal RM, Sahota A (1997). New concepts in medicine: The emerging role of cell migration and chimerism in the induction of tolerance after transplantation. Postgrad Med J 73: 146-150.

2. McDaniel HB, Sidner RA, Yang M, Burton M, Shao C, Tischfield JA, Jindal RM, Sahota A (1997). Evaluation of a mouse model for the study of microchimerism and tolerance. Surg Forum 48: 510-511.

3. Sahota A, Yang M, Sidner RA, McDaniel HC, Book B, Barr R, Brahmi Z, Jindal RM (1998). Evaluation of seven PCR-based assays for the analysis of microchimerism. Clin Biochem 31: 641-645.

4. McDaniel HB, Yang M, Sidner RA, Jindal RM, Sahota A (1999). Prospective study of microchimerism in transplant recipients. Clin Transplant 13: 187-192.

5. Sahota A, Yang M, McDaniel HB, Hall M, Sidner RA, Jindal RM (1999). Microchimerism analysis using PCR assays that selectively amplify donor DNA. Transplant Proc 31: 800-801.

6. Sahota A, Gao S, Hayes J, Jindal RM (2000). Microchimerism and rejection: A meta-analysis. Clin Transplant 14: 346-351.

7. Brooks AI, Viale A. (2003). Assessing, understanding and minimizing variability of DNA microarrays. Am Pharm Rev. 6:102-105.

8. Bijlani R, Cheng Y, Pearce DA, Brooks AI, Ogihara M. (2003). Prediction of biologically significant components from microarray data: Independently Consistent Expression Discriminator (ICED). Bioinformatics. 19: 62-70.

9. Sahota A, Brooks AI, Wilde L, Wang Q, Fugman DA, Toke DA, Tischfield JA (2006). Whole genome amplification of degraded DNA samples. J Molec Diagnos 8: 672 (abstract).

10. Viale A, Li J, Tiesman J, Hester S, Massimi A, Griffin C, Grills G, Khitrov G, Lilley K, Knudtson K, Ward B, Kornacker K, Chu CY, Auer H, Brooks AI. (2007). Big results from small samples: evaluation of amplification protocols for gene expression profiling. J Biomol Tech. 18:150-61.

11. Sahota, A., Brooks, A. I. and Tischfield, J. A. (2007a). Preparing DNA from Mammalian Sources; in Genetic variation: a laboratory manual, Weiner, M. P., Gabriel, S. and Stephens, J. C. (eds.), pp. 103, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

12. Sahota, A., Brooks, A. I. and Tischfield, J. A. (2007b). Protocol 1: Preparing DNA from Cell Pellets; in Genetic variation: a laboratory manual, Weiner, M. P., Gabriel, S. and Stephens, J. C. (eds.), pp. 107, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

13. Sahota, A., Brooks, A. I. and Tischfield, J. A. (2007c). Protocol 6: Preparing DNA from Blood: Large-Scale Extraction; in Genetic variation: a laboratory manual, Weiner, M. P., Gabriel, S. and Stephens, J. C. (eds.), pp. 124, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

14. Sahota, A., Brooks, A. I. and Tischfield, J. A. (2007d). Protocol 7: Preparing DNA from Saliva; in Genetic variation: a laboratory manual, Weiner, M. P., Gabriel, S. and Stephens, J. C. (eds.), pp. 129, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

15. Sahota, A., Brooks, A. I., Tischfield, J. A. and King, I. B. (2007a). Protocol 5: Preparing Genomic DNA from Whole Blood: Small- and Mid-Scale Extraction; in Genetic variation: a laboratory manual, Weiner, M. P., Gabriel, S. and Stephens, J. C. (eds.), pp. 120, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.