Skip to main content
Home
  • About Us
    • About Us
    • Mission & Values
    • Leadership
    • News
    • Careers
    • Partnerships
    • Support
    • Achievements
  • For Researchers
    • Clinical Trials
      • Allergy & Asthma
      • Autoimmune Disease
      • Transplantation
      • Type 1 Diabetes
    • Core Facilities
    • Lab Protocols
      • ITN Protocols
      • T1D Preclinical Consortium
    • Policies
      • Publications Policy
    • Proposals
      • Submit Proposal
      • Current RFPs
      • Past RFPs
    • Publications
    • TrialShare
    • Strategic Plans
      • Allergy
      • Autoimmune Disease
        • Type 1 Diabetes
      • Transplantation
  • For Patients
    • Allergy & Asthma
    • Autoimmune Disease
    • Transplantation
      • Kidney
      • Liver
    • Type 1 Diabetes
    • Clinical Trial Info
  • TrialShare
  • Contact
    • Contact Us
      • BRI - Seattle Office
      • UCSF - San Francisco Office
    • Media Inquiries
Home

Secondary links

  • Home
  • TrialShare
  • Member Portal
  • About Us
    • About Us
    • Mission & Values
    • Leadership
    • News
    • Careers
    • Partnerships
    • Support
    • Achievements
  • For Researchers
    • Clinical Trials
    • Core Facilities
    • Lab Protocols
    • Policies
    • Proposals
    • Publications
    • TrialShare
    • Strategic Plans
  • For Patients
    • Allergy & Asthma
    • Autoimmune Disease
    • Transplantation
    • Type 1 Diabetes
    • Clinical Trial Info
  • TrialShare
  • Contact
    • Contact Us
    • Media Inquiries

Primary links (clinical trials)

  • About Us
    • About Us
    • Mission & Values
    • Leadership
    • News
    • Careers
    • Partnerships
    • Support
    • Achievements
  • For Researchers
    • Clinical Trials
      • Allergy & Asthma
      • Autoimmune Disease
      • Transplantation
      • Type 1 Diabetes
    • Core Facilities
    • Lab Protocols
      • ITN Protocols
      • T1D Preclinical Consortium
    • Policies
      • Publications Policy
    • Proposals
      • Submit Proposal
      • Current RFPs
      • Past RFPs
    • Publications
    • TrialShare
    • Strategic Plans
      • Allergy
      • Autoimmune Disease
      • Transplantation
  • For Patients
    • Allergy & Asthma
    • Autoimmune Disease
    • Transplantation
      • Kidney
      • Liver
    • Type 1 Diabetes
    • Clinical Trial Info
  • TrialShare
  • Contact
    • Contact Us
      • BRI - Seattle Office
      • UCSF - San Francisco Office
    • Media Inquiries

High-Throughput Immunosequencing Core

Principal Investigator

Harlan Robins | Adaptive Biotechnologies | Seattle, WA

Abstract

Adaptive Biotechnologies performs immune profiling of T cell and B cell receptors using ImmunoSEQ technology. The ImmunoSEQ system combines the capabilities of ultrahigh-throughput DNA sequencing with a proprietary multiplex PCR methodology and a powerful bioinformatics software suite to provide exceptionally deep access to T cell and B cell repertoires.

The laboratory at Adaptive Biotechnologies, managed by Dr. Robert Livingston and Dr. Mark Reider, is currently undergoing CLIA certification. The laboratory is equipped with 2 Illumina HiSeq platforms and 1 Illumina MiSeq platform to accommodate client samples.

The protocol for T cell receptor sequencing remains unchanged since the laboratory started receiving samples for the Immune Tolerance Network (ITN) study. Quality control samples (positive and negative) are processed with every multiplex PCR and high-throughput sequencing run. From November 2011 to April 2012, this core received 142 ITN DNA samples for TCRβ sequencing. All 142 DNA samples were successfully sequenced on the Illumina HiSeq platform and analyzed using the ImmunoSEQ analyzer software.

Adaptive Biotechnologies will continue to perform ultrahigh-throughput DNA sequencing as needed for commercial and research clients. Currently, the laboratory sequences human TCRβ, TCRγ and IgH, and mouse TCRβ. In the future, the laboratory plans to add a TCRα assay for humans.

 

Principal Investigator

Harlan Robins | Adaptive Biotechnologies | Seattle, WA

The Immune Tolerance Network and is sponsored by the National Institute of Allergy and Infectious Diseases, part of the National Institutes of Health.

National Institute of Allergy and Infectious Diseases

Footer

  • Home
  • Sponsors
  • Careers
  • Contact
  • Search

© 2021 Immune Tolerance Network

  • Privacy
  • Terms of Use
  • Accessibility